International Journal of Microbiology and Immunology Research Vol. 2(6), pp. 075-091, October 2014 ISSN 2327-7769 ©2014 Academe Research Journals

Full Length Research Paper

Bioconversion of coffee husk for oyster mushroom (Pleurotus ostereatus) cultivation in Jimma

Seid Mohammed¹*, Diriba Muleta² and Dawit Abate²

¹Department of Biology, Adama Science and Technology University, Ethiopia,

²Department of Biology, Addis Ababa University, Ethiopia.

*Corresponding author. E-mail: seidmohes372@gmail.com.

Accepted 16 September, 2014

Abstract

At present, coffee production is dramatically increasing in the world. Consequently, coffee husk are also increasing at the same time. In order to minimize this hazardous husk, the current study was initiated to evaluate the suitability of coffee husk for cultivation of commercial oyster mushroom species (Pleurotus ostreatus) after compositing with different main substrate combinations. Composting of coffee waste (husk) was conducted with cow dung, poultry manure and bone meal in the ratio of 3:1. During cultivation of mushroom, some biological efficient and other parameters were conducted. Data were analyzed using SPSS version 16. The substrate combinations of coffee husk showed a significant variation (p<0.05). The highest yield (192.3 g) and biological efficiency (21.37%) was obtained from combination A on 20 days composting. Nevertheless, the lowest biological efficience was obtained from combination of A + B (7.32%) on the first 30 days of composting. No variation was observed between combination A and CH (P>0.05) in terms of yield on the first 5 and 10 days of composting. But, great variations (p<0.05) were generally detected between combinations A+B and A, and A+B and CH. Therefore, better yield of oyster mushroom was obtained after bioconversion of this cost-effective and cheap agro-waste of coffee husk.

Key words: Coffee husk, compost, microorganism, oyster mushroom, substrate combination.