Academe Research Journals

International Journal of Biotechnology Research

Full Length Research Paper

Genetic variability and molecular characterization of F2 population of soybean, Glycine max (l.) Merrill genotypes using SNP markers

¹*Adewusi Olamide Folake and ²Fayeun Lawrence

¹Department of Biotechnology, Federal University of Technology, Akure, Ondo State, Nigeria

²Department of Crop, Soil and Pest Management, Federal university of technology, Akure

*Corresponing author. E-mail: ofadwusi@gmail.com, lawrencefayeun@yahoo.com . Tel.: 08101109482

Accepted 11 January, 2020

Abstract

This study was carried out on the genetic variability and molecular characterization of F2 population of soybean, Glycine max (l.) Merrill genotypes using SNP markers. The aim of the study was to assess the magnitude of genetic variability, heritability and genetic advance and estimating the genetic diversity among the population using SNP markers. The field experiment was laid out in a randomized complete block design (RCBD) with three replications. The mean performance and genetic components of yield and its related characters were estimated as well as the genetic diversity among the F2 populations using SNP markers. The results showed that TGx1835–40E x TGx1830–20E(A), TGx1830–20E x TGx1990–57F(B) and TGx1990 – 3F x TGx1990–37F(B) recorded the shortest days to flowering while TGx1835–40E x TGx1830–20E(A) and TGx1835–40E x TGx1830–20E(B) recorded the highest mean values for seed yield. Heritability in the brad sense ranged from 40.00% to 99.97% for number of seeds and number of pods respectively. The difference between PCV% and GCV% ranged between 0.18 and 15.87 for number of pods and number of seeds respectively. The genetic advance mean ranged from 10.03 to 130.17 for days to maturity and seed yield respectively. At the molecular level, SNP markers were used to assess the extent of polymorphism among the F2 populations. The markers showed remarkable genetic diversity.

Key words: Genetic variability, molecular characterization, F2 population, soybean genotypes, SNP markers